LOCUS       40924_26784        3267 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pKITE301 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Toda, H., Itoh, N.
  TITLE     Development of novel E. coli-Kocuria shuttle vector using cryptic 
            plasmid pKPAL3 from Kocuria palustris IPUFS-1 and utilization for 
            producing enantiopure (S)-styrene oxide
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Itoh N, Toda, H.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-AUG-2017) Contact:Nobuya Itoh Toyama Prefectural 
            University, Biotechnology Research Center; 5180 Kurokawa, Imizu, 
            Toyama 939-0398, Japan URL :http://www.pu-toyama.ac.jp/BR/itoh/
REFERENCE   3  (bases 1 to 3267)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3267)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-AUG-2017) Contact:Nobuya Itoh Toyama Prefectural University, 
            Biotechnology Research Center"; volume: " 5180 Kurokawa, Imizu, 
            Toyama 939-0398, Japan URL :http"; pages: 
            "//www.pu-toyama.ac.jp/BR/itoh"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    39..60
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        75..105
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    113..129
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     137..153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    162..218
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(222..238)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(460..1272)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1654..2242
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      2264..3267
                     /label=pKPAL3-derived plasmid replication origin
                     /note="pKPAL3-derived plasmid replication origin"
ORIGIN
        1 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
       61 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      121 ggataacaat ttcacacagg aaacagctat gacatgatta cgaattcgag ctcggtaccc
      181 ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg cactggccgt cgttttacaa
      241 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
      301 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
      361 agcctgaatg gcgaatgcga tttattcaac aaagccgccg tcccgtcaag tcagcgtaat
      421 gctctgccag tgttacaacc aattaaccaa ttctgattag aaaaactcat cgagcatcaa
      481 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
      541 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
      601 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
      661 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
      721 cttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
      781 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg
      841 atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
      901 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
      961 tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
     1021 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
     1081 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
     1141 atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
     1201 atataaatca gcatccatgt tggaatttaa tcgcggcttc gagcaagacg tttcccgttg
     1261 aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca
     1321 tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct
     1381 ttgttgaata aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa
     1441 cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc aactggtcca cctacaacaa
     1501 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcctg
     1561 gtatgagtca gcaacacctt cttcacgagg cagacctctc gacggatcgt tccactgagc
     1621 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1681 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1741 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1801 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1861 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1921 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1981 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2041 tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2101 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2161 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     2221 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttagatctt tcgctcgtgc
     2281 tgttctttga ggccctgccc tccgccttgc tgccttcgtg ctgtgctcgg gatctcgagc
     2341 gcaacctccg accagcctgg gagggaagga gggagcggag ggcagggcga tgccttggac
     2401 gccccgagga cacccgggcg gggtgtggtg gggacgttca aggcatcgtc aatcggtggt
     2461 gcgggaccag cccggtcggt cggtcggtcg catcggttgg ccaccgttgg tcacgcaccg
     2521 tgaggggtgc gtggggtggc tgacaacggc tcagggcgtt actcctgtgc agtggcggca
     2581 tgtgcgtgca gctctggtca cacgctcggg gcagggctgc tgtctcccga gggcggctgt
     2641 agggcttggc actccttcgc tgtccgggga ttgctcgacc cggtgacggc ccctggctgc
     2701 acgctcggca aaagaaccgg ctctgacccg ggaggggccg cgaaggcagt gcctcacaag
     2761 cggctggcga tgttggttca cggggcttcg catcccgacg ccagggtgtt ccgatcgctg
     2821 gcgggtccag ttgcaggccg ttggcgcggc ggtagactgg tctcggtatt tactacagac
     2881 ctcatggtag gcccccgttg gcgcgggggc ctaccgcata cctagggggg cgtgtcgtca
     2941 gacacgcccc ctaggtgttg tggggcatga ggttcttgtc accagcggcg cgctgagatg
     3001 aggcgagcgg gctcctcgtc acaggatgga agttcctcaa caagccatcc acggaagaca
     3061 gagcccgctc atgccccacg ctaagcgaag ttttctacga tgcagtagat cccgcagggt
     3121 acgtaagtag aacaattcag cacatcgcca gtcatgcctt cacccctagt gaggtgcgtc
     3181 agcttttaga aacctccggc ttcaagaata ttgcaatgta tgggggtttt tctttcggcc
     3241 ccctaactga ttcaagtttc gccatgg
//
